抄録
A simple direct colorimetric method was developed for the quantification of serum total bile acids. Two tenth ml of serum was mixed with 10g/L oxamic acid solution. After 5min. incubation at room temperature, to the solution were added 3x-Hydroxy steroid: NADoxidoreductase (3α-HSD), NAD, Diaphorase and Nitrotetrazolium Blue(NTB). The mixture was incubated at 37° for 8min, then the color of resultant Formasan was measured at 540nm.
A linear relationship was obtained between the amount of bile acids and the optical densities in the range 1 to 100μmol/L. The recovery of bile acids added to the serum was 91±10%. The precision of day to day was 10μmol/L with standard deviation of±0.50 and C. V. of 5.0%. The concentration of bile acids in normal fasting sera was less than 6μmol/L.
