Abstract
A noble system for estimating HDL-cholesterol by a continuous ultrafiltration method is described. Immunocomplexes, produced after reaction between Apo β-associated lipoprotein in sample serum and anti β-lipoprotein goat serum, form gradually large precipitates in a mixing coil and are removed by a membrane filter attached to a dialyzer.
The immunocomplex-free HDL fraction is continuously mixed with enzymic reagent to start cholesterol determination. The day-to-day precision of this system is fairly good (±CV% of 2.75 to 4.36%). Correlation of this system with the airfuge ultracentrifugal procedure was studied by the use of least squares regression analysis, and we obtained the following values: n=55, y=0.89x+3.7, r=0.978.
Our data indicates that continuous flow system for the estimation of HDL-cholesterol can be used efficiently for routine tests.
