A New Sensitive Assay Method for Kallikrein-Like Arginine Esterases Using N-α-Carbobenzoxy-L-Lysine Thiobenzyl Ester (ZLSB)

Abstract

A simple and sensitive assay method for kallikrein-like arginine esterases using N-α-carbobenzoxy-L-lysine thiobenzyl ester (ZLSB) as substrate was established. The present method was sixteen thims more sensitive than the spectrophotometric method using N-α-benzoyl-L-arginine ethyl ester (BAEE) as substrate. Minimum identification limit of the enzyme activity in the present method was recognized as 0.95 n mole of substrate hydrolyzed per minute as enzyme activity judging from the minimum value in the present expreiment.