Assay of LDH Isoenzyme with High Performance Liquid Chromatography (HPLC).

Abstract

1) With use of high performance Liquid chromatography (HPLC) and continuous flow fluorometric monitoring of LDH, LDH isoenzymes were assayed within 7min in 10μl of human serum.
2) HPLC method was more rapidly and easily performed than electrophoretic method, and its reproducibility and quantitativity were sufficient for utilization in clinical chemistry. This method was favorable for LDH assay, because LDH reaction was conducted in a short time such as 8min at 37°C, and in a homogeneous solution.
3) A good correlation was obtained between HPLC method and elecrophoretic method. However, some problems of this method are remained to be solved such as an appearance of a minor LDH peak usually eluted before LDH-3, and the effect of coexistent substance in serum on the fluorometric LDH assay.