Abstract
In this study, application of immobilization of E. coli JM109 (pUCDEXL) introduced recombinant plasmid DNA coding 2-halo acid dehalogenase gene from Burkholderia cepacia KY and its effect on the microbial activity were discussed. E. coli JM109 (pUCDEXL) was immobilized with agar, polyvinyl alcohol and acrylamide. The activity was comparatively higher, but some leakage of E. coli JM109 (pUCDEXL) was found in case with polyvinyl alcohol. Although the remaining activity was rather lower after immobilization, the cell was sufficiently held in acrylamide. Immobilization was effective in the reaction at pH8-10. Dehalogenase activity in immobilized E. coli JM109 (pUCDEXL) sustained longer than free cell. These results suggested the possibility that immobilization could improve stability of genetically engineered microorganisms for bioremediation.
