PATTERNS OF UNCULTURED BACTERIA PHYLA IN DIFFERENT WASTEWATER TREATMENT SLUDGES

Abstract

 Comprehensive understanding of biological wastewater treatment mechanisms was prevented because a wide range of uncultured and unknown lineages existed in the wastewater treatment sludges. In this study, therefore, to understand the patterns of uncultured phyla in wastewater treatment sludges, we analyzed a total of 54 aerobic, anoxic and anaerobic sludge samples collected from 17 different wastewater treatment reactors by massively parallel 16S rRNA gene sequencing. We analyzed total of 1, 041, 539 sequence reads of 16S rRNA gene. The results of microbial community analysis at the phylum level and phylogenetic diversity analyses indicated that patters of microbial communities depended heavily on types of wastewater and types of treatment technologies. We could estimate the putative habitats and environmental conditions of these uncultured lineages by the distribution pattern of the microbial communities in different wastewater treatment sludges.