Abstract
Effluent tests of secondary effluent with high concentrations of dissolved metals were conducted based on biological responses of Ceropdaphnia dubia and Daphnia magna. The secondary effluent affected the survival and reproduction of C. dubia but did not affect the survival and reproduction of D. magna. Results indicate that C. dubia was more sensitive to the secondary effluent than D. magna. Toxicity identification evaluation tests indicated cation metals and ammonia as substances that caused effects of the secondary effluent on C. dubia. Effects of cation metals and ammonia on C. dubia were evaluated based on results of water quality analysis. Results showed the effect of nickel included in the secondary effluent on C. dubia. Toxicity identification test could detect the biological effect of dissolved cation metals in the secondary effluent used in this study.