Abstract
Investigation of the influence on soil animal by chemical agents is important. OECD provides the test guidelines of acute toxicity test 207 (TG 207) using Eisenia fetida (earthworm).
We performed 7-day acute toxicity tests using the artificial soil in reference to TG 207, and in this study tested three kinds of chemical agents such as Sumithion of agrochemical agent, Portland cement of soil improvement material, Calcium chloride of the melting snow agent. As a result, median lethal concentration (LC50) for Eisenia fetida of 7-day was 0.418 mg/kg of Sumithion, 19.6 g/kg of Normal Portland cement, 22.1 g/kg of Calcium choloride. The cause of death is thought about each case, in sumithion Eisenia fetida was direct influence with the agent component, in Normal Portland cement they died by solidification and increase in pH, in Calucium chloride they died by water absoption. In addition, we compared the test result and the estimate concentration of each chemical agent spread on the soil.
