Abstract
We laid out the waste oyster shells in a closed harbor area on the western part of Bisan Seto, and researched the change of biota. Various organisms increasing since two months later, they have reached 117 kinds (2.2 times of the control), 4,700 inds/m2 (2.3 times) and 487.3 g/m2 (13.2 times) for 1.5 years. Resolved carbon was calculated at 114.2 kgC/year/1,000 m2. The shells layer kept to be visible, not buried, at sea floor in this period. These results are suggested that the biological activities can make diversity and biomass in the shells layer increase, and keep not covering with organic particles.