Abstract
High molecular weight glycoprotein (HMWGP) isolated from bovine red blood cells (BRBC) contains two antigenic determinants of H-D complexes. One is N-glycolyl neuraminic acid (NGNA) ganglioside, of which antigenic determinants are destroyed by treatment with neuraminidase and another is asialo-HMWGP, of which antigenic activities are resistant to neuraminidase. Using HMWGP and asialo-HMWGP as antigen, H-D antibody activities in sera of patients with liver diseases were examined by enzyme immunoassay (EIA) and trypsinized BRBC hemagglutination.
Four of ten sera showed high O. D. values of antibodies in EIA against both HMWGP and asialo-HMWGP and BRBC hemagglutinating activities in these sera were similarly inhibited by these two antigens. On the other hand, two sera showed extremely low O. D. values against asialo-HMWGP but high O. D. values against HMWGP. Subsequently, hemagglutination activites of these two sera were inhibited by HMWGP but not by asialo-HMWGP.
The results would indicate that antigenic determinants to which H-D antibodies in liver disease sera reacted were asialo-HMWGP and/or NGNA ganglioside.
