1987 Volume 10 Issue 1 Pages 66-70
The effect of Platonin on human peripheral blood lymphocytes (PBL) was studied in vitro. Platonin showed proliferative effect on PBL. When PBL was stimulated with T cell mitogen Phytohemagglutinin (PHA) and B cell mitogen Staphylococcus aureus Cowan I (Cowan I), it showed enhancing effect on the former and suppressive effect on the latter. In Pokeweed mitogen (PWM) induced immunoglobulin (Ig) productive system, both of the T.B cells, T cells only, or B cells only were pretreated with Platonin and then co-cultured. The Ig secreting cells were demonstrately decreased than that of the absent Platonin pretreated in any groups. However, the 3H-thymidine incorporation failed to give a significant variation in any groups. In the experiment of T cell subsets detected with monoclonal antibodies and by Fluorescent Activaed Cell Sortor (FACS), the OKT8+ T cells showed a high proportion in the Platonin treated groups. In summary, B cells were directly suppressed by Platonin and concerning T cells, the T cell subsets showed a high proportion of suppressor/killer T cells. So it is suggested that Platonin suppressed Ig prodution through suppressing B cells and enhancing suppressor/killer T cells.
