Abstract
Changes in cytotoxic activity were studied in lymphocytes cultured with the addition of tumor cells treated to preserve tumor antigenicity. AH109A cells were treated with (1) Mitomycin C, (2) 3M KCl, (3) ultrasound and (4) lyophilization and these treated cells were added to culture systems of lymphocytes from Donryu rats. The lymphocytes cultured with lyophilized antigen resulted in the highest cytotoxic activity.
Human lymphocytes cultured with lyophilized autologous ascitic cancer cells demonstrated a higher cytotoxicity against the same cancer cells than the corresponding cells cultured without the antigens.
In the cross reactive test using BALB/c mouse lymphocytes and several kinds of tumor cells, lymphocytes cultured with lyophilized antigen demonstrated an antigen-specific cytotoxicity.
This data suggests that autoimmunotherapy using lymphocytes cultured with lyophilized tumor cells appears to be clinically useful for cancer therapy.
