Abstract
We measured anti-nuclear antibody (ANA) by the method of enzyme-linked immunosorbent assay (ELISA) in 150 cases with collargen disease and evaluated its clinical usefulness in comparison to the indirect immunofluorescence antibody method (IF). ELISA-ANAs with three different types of antigens were especially examined. When the cut off values were set at 3.0 units ELISA-ANA and 20 dils IF-ANA, the sensitivity, specificity and coincidence were 82.1%, 80.0%, 82.0% (IF vs ANA-I ), 88.6%, 70.0%, 87.3% (IF vs ANA-II), 97.9%, 66.7%, 96.0% (IF vs ANA-III), respectively. The measured values by ANA-III were significantly higher than those by the other types (p<0.01). The correlation cofficients between ELISA-ANA-III and IF-ANA in SLE, Primery SjS and PSS; NCTD; PM/DM were r=0.618, 0.591 and 0.682, respectively.
The case with anticentromere or anti PCNA antibody positive by IF-ANA could be detected only by ELISA-ANA-III among ELISA.
We conclude that ELISA-ANA-Ill is a useful screening test for total ANA detection.
