Abstract
The proportion of CD4/CD8 T-cell subsets and HLA-DR and IL2 receptor (IL2R)-expression on peripheral T-lymphocytes from patients with infectious mononuleosis (IM) were examined.
There was a significant increase in both the proportion and the absolute number of CD8 positive (CD8+) cells in comparison with those in healthy adults; thus the ratio of CD4+ cells to CD8+ cells was significantly decreased. HLA-DR expression on T-cells, particularly on CD8+ cells, was increased. The expression of IL2Rα (CD25) was not increased, but that of IL2Rβ was increased. The number of CD7+ cells decreased and that of CD45RO+ CD8+ cells increased. These findings were thought to represent the activation of CD8+ T cells and the increase of memory T cells.
We found a significant increase of TdR-incorporation of the lymphocytes in IL2 stimulation in contrast to PHA stimulation. By IL2 stimulation for 48h, the number of HLA-DR+ CD8+ cells was increased as compared with medium alone and the proliferating cells were CD7+ CD8+, though most of the fresh cells from patients were CD7- CD8+. And after PHA stimulation for 48h, the fluorescence intensity of CD7 increased and HLA-DR- cells were changed into HLA-DR+ cells. After incubation for 48h, the number of viable cells from patients with IM was lower than that from healthy adult volunteers.
