Studies on the idiopathic thrombocytopenic purpura

2. The in vivo localization of anti-platelet antibody on the bone marrow megakaryocytes and its IgG subclass and in vitro IgG production by bone marrow and splenic lymphocytes due to pla

Abstract

The in vivo localization of anti-platelet antibodies on the bone marrow megakaryocytes and its subclass were studied in SLE and ITP by means of the peroxidase labeled antibody method. The in vivo IgG production by bone marrow and splenic lymphocytes by platelets stimulations was also examined in ITP and SLE.
The in vivo localization of IgG on the bone marrow megakaryocytes was positive in 5 out of 6 patients with ITP. Its subclass was IgG₃. The in vivo localization of IgG was demonstrated only in one of 7 patients with SLE and its subclass was IgG₁. Bone marrow and splenic lymphocytes produced IgG in vivo by the stimulation of platelets.