Partial purification of the cholestatic factor prepared from PPD-activated human lymphocytes

Yasuhiro Mizoguchi; Yoshihide Sakagami; Toshihiro Higashimori; Fumiaki Ohnishi; Masahiro Nakao; Takeyuki Monna; Sukeo Yamamoto; Seiji Morisawa

doi:10.2177/jsci.4.305

Yasuhiro Mizoguchi, Yoshihide Sakagami, Toshihiro Higashimori, Fumiaki Ohnishi, Masahiro Nakao, Takeyuki Monna, Sukeo Yamamoto, Seiji Morisawa

Author information

Yasuhiro Mizoguchi
The Third Department of Internal Medicine, Osaka City University Medical School
Yoshihide Sakagami
The Third Department of Internal Medicine, Osaka City University Medical School
Toshihiro Higashimori
The Third Department of Internal Medicine, Osaka City University Medical School
Fumiaki Ohnishi
The Third Department of Internal Medicine, Osaka City University Medical School
Masahiro Nakao
The Third Department of Internal Medicine, Osaka City University Medical School
Takeyuki Monna
The Third Department of Internal Medicine, Osaka City University Medical School
Sukeo Yamamoto
The Third Department of Internal Medicine, Osaka City University Medical School
Seiji Morisawa
The First Department of Biochemistry, Oska City University Medical School

Keywords: lymphokine, cholestatic factor

JOURNAL FREE ACCESS

1981 Volume 4 Issue 6 Pages 305-311

DOI https://doi.org/10.2177/jsci.4.305

Details

Abstract

The cholestatic activity which induces a marked reduction of bile flow was detected in the activated lymphocyte culture fluid when the peripheral blood lymphocytes from patients with pulmonary tuberculosis were stimulated with PPD in vitro. This factor (or factors) was fractionated by Sephadex G-75 and DEAE-cellulose column chromatography and was shown to have a similar property as guinea pig cholestatic factor.

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