Effect of streptococcal preparation, OK-432 on cultured spontaneous cell mediated cytotoxicity (CSCMC) and Ig production in vitro

Abstract

The effect of the steptoccocal preparation OK-432 on the in vitro immune responses by the human periperal blood lymphocytes was studied.
The results obtained were as follows.
1) OK-432 did not enhance natural killer (NK) cell activity.
2) OK-432 enhanced cultured spontaneous cell mediated cytotoxicity (CSCMC). The most enhancing effect is at a concentration of around 10^-3 KE/ml and is recognized as statistically significant at the cultured duration of 3, 5 and 7 days.
The activity of CSCMC was most remarkable in the cell fraction which was not adherent to nylon wool and not rosetted with En.
However, some of En-rosette-forming cells also had a little activity.
3) OK-432 induced much more Ig production by B cell than did PWM.
4) OK-432 stimulated DNA synthesis of lymphocytes.