Abstract
In this study we intended to Characterized the Ia-antigen positive (Ia+) T cells in peripheral blood from normal individuals, detecting by indirect antiglobulin rosetting assay utilizing monoclonal anti-human DR antibody, which reacted with the human DR antigen framework.
Higher percentage of Ia+ T cells than those described previously by using indirect immunofluorescence was observed in normal peripheral blood. After PHA, Con A or PWM activation, the proportion of Ia+ T cells increased up to about 30% of T cells. It also rose rapidly after inoculation with PPD in sensitized individuals only. The proportion of Ia+ T cells well correlated with the proportion of Tr cells. It was also revealed that Ia+ T cells were enriched in the OKT+4 cell-rich population than OKT+8 population from T cells of normal unstimulated individuals.
