In dicotyledonous plants including Arabidopsis, brassinosteroids deficiency induces morphological features of light-grown plants in the dark, i. e. short hypocotyls, expanded cotyledons, and true leaves, in a dose-dependent manner. While how brassinsteroid deficiency does affect the growth of monocotyledonous plants in the dark is still not clear. In order to investigate the effect brassinosteroid deficiency in monocotyledonous plants, a brassinosteroid biosynthesis inhibitor was applied to rice in the dark and the morphological change was intensively observed and the expression of genes induced under light condition was examined.
