Detection of HLA class I antibodies in WAKFlow ^Ⓡ HLA antibodies Class I (HR)

Abstract

【Objective】For detection of Donor Specific Antibodies (DSA), solid phase immunoassays (SPI) have been used widely；however, reactions with serum samples are ambiguous among commercial reagents. We evaluated the results produced by two antibody detection assays：LABScreen^Ⓡ Single Antigen (LSSA) and WAKFlow^Ⓡ HLA Class I (HR).【Method】We compared the results between LSSA and HR on 24 serum samples where LSSA detected HLA antibodies. The antibodies of interest were 43 HLA antibodies that are shared between LSSA and HR. The total number of assayed antibodies in this study was 1,032.【Result】The concordance rate for LSSA and HR was 94.7％. There were 31 cases (antibodies) where LSSA showed a positive reaction whereas HR yielded a negative one. On the other hand, there were 24 cases where LSSA showed a negative reaction, but HR produced a positive result.【Conclusion】There are mismatches between the results of LSSA and HR for a number of antibodies. For detection of DSA, serological methods in addition to SPI may be necessary.