Abstract
This review describes the analytical techniques for evaluating the structural phase transition of a small protein. First, it outlines the significance of thermal analyses in protein research. Next, a practical limitation of micro DSC to detect the structural phase transition of a small protein is mentioned, though this method is adequate for ordinary proteins. We then introduce the strategy for analyzing a small protein combining the optical measurements using chemometrics and the atomic level analyses by NMR, which were conducted by us to overcome the above problem. Finally, the meaning of the thermodynamic result obtained from these analyses is discussed.
