Fluorescein Angiography of the Ocular Fundus in Rats with a New Portable Ophthalmoscopic Camera

Abstract

Fluorescein angiography of the ocular fundus in rats was performed using a new portable ophthalmoscopic camera (Genesis; Kowa Optimed. Inc.). Seventeen Wistar male rats weighing 200-500 g, were used under general anesthesia. Ten percent fluorescein solution was injected intravenously into the external jugular vein with a disposable winged infusion set. The angiogram was subdivided into three major phases, i.e., the retinal arterial phase, the retinal arteriovenous phase, and the retinal venous phase. In these albino rats the choroidal phase could not be recorded. However, this technique of fluorescein angiography could be useful in assessing cinephotographic measurement of retinal circulation time in rats as well as in other mammals.