Ophthalmoscopic Examination of Ocular Fundus in Rats, Mice and Rabbits
1995 Volume 14 Issue 3-4 Pages 3-4_115-3-4_124
Details
1995 Volume 14 Issue 3-4 Pages 3-4_115-3-4_124
Fundus photography (FP) and fluorescein angiography (FA) were performed using a fundus camera, Kowa RC-2 Model-621 for small animal use in rats and mice and Kowa RC-2 for veterinary use in rabbits.
FP in rats and FP and FA in rabbits were performed in a conscious state without anesthesia; FA in rats and FP and FA in mice, under general anesthesia.
The appropriate conditions for fundus photography were: 50W lighting at Flash No. 4 in albino rats and mice: and 50W lighting at Flash No. 2 in rabbits. The fundus photography in albino rabbits required a neutral density filter (ND-0.6), because an intensified retinal reflex interferred with photographing. On the other hand, in pigmented rats and mice, good photographs were obtained with 100W lighting.
The suitable conditions for fluorescein angiography were: injection of 0.4ml/kg of 10% fluorescein under 100W lighting in albino rats; 0.3ml/kg of 10% fluorescein under 100W lighting in pigmented rats; and 0.2ml/kg of fluorescein under 50W lighting at Flash No. 6 in albino or pigmented rabbits.
