Immunohistochemical demonstration of advanced glycation end products in synovial amyloid deposits of patients on long-term hemodialysis

Abstract

β₂-Microglobulin (β₂M) isolated from the amyloid tissue of patients with dialysis-related amyloidosis (DRA) has been reported to be modified by advanced glycation end products (AGE) via the Maillard reaction. We produced anti-AGE monoclonal antibody and used it to immunohistochemically localize AGE in amyloid tissue obtained during surgical operations on patients with carpal tunnel syndrome (CTS). AGE was found to be localized in β₂M-positive amyloid tissues. AGE was also detected in infiltrating cells surrounding the amyloid tissue. In conclusion, β₂M-positive amyloid tissue was immunohistochemically demonstrated to be modified by AGE.