2000 年 59 巻 4 号 p. 298-305
Using an infrared charge-coupled device (CCD) camera for recording simple eye movement is widely accepted but since quantitative analysis of the video image produced is still difficult, we devised a new eye movement image analysis technique using a personal computer. The analysis was performed using the public domain NIH Image program (developed by the U.S. National Institutes of Health, http://rsb.info. nih.gov/nih-image/). The video image from an infrared CCD camera was captured at 30 frames per second in 320 × 240. Using the original macro, the X-Y center of the pupil was automatically analyzed, and the horizontal and vertical components were calculated. The merits of this technique are: (1) it is easy to use and very low-cost; (2) many types of infrared CCD cameras are available; (3) there are cross-applications to animal experiments; (4) it is possible to make a detailed analysis of eye movements such as the rotation axis of the eyeball.