Temperature Dependence of Retention Factor of Saccharides on Hydrophobic Resin

Abstract

Retention factors of galactose, glucose, trehalose, and maltotriose for a column packed with hydrophobic resins were measured using water as the eluent in the temperature range of 0.5 to 150℃. The van’t Hoff plots of the retention factors were nonlinear, indicating that hydrophobic interactions between the resin and solutes occurred. The temperature dependence of the retention factor could be analyzed based on Kirfhoff’s law and the enthalpy change for distribution was calculated.