Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Short Papers
Study of Simple and Rapid Detection of Salmonella spp. Using VIDAS
Second Report; The New Protocol with Unique Enrichment Broth
Naoko NAKAZATOYoshiaki YABIKUYuko UEMAKeisuke FUKUMURAKazuyuki UCHIDASusumu SAWAGUCHIHiroshi NAKAGAWA
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2007 Volume 24 Issue 4 Pages 178-182

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Abstract

In our previous report, we evaluated and reported some VIDAS protocols for the detection of Salmonella. In this report, we evaluated the new additional protocol “Easy SLM” with “SX broth”, a new unique enrichment broth. This method needs about 2 days to complete the total protocol, 1-2 days less than the conventional culture method. We tested 77 food samples with and without artificial inoculation with Salmonella strains. The positive rate for each method of testing food samples with and without artificial inoculation of Salmonella strains were almost the same, and as a result, the sensitivity and specificity of the Easy SLM protocol and the conventional culture method were equal. Therefore, the Easy SLM protocol is useful for routine quality control for the detection of Salmonella because of its rapidity, ease of handling and automation for standardization.

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© 2007 Japanese Society of Food Microbiology
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