Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Short Paper
Evaluation of Quickly Campylobacter jejuni/coli Detection Method Using “Campylo-Preston/225” and “Cica-Immunotest Campylobacter II”
Akira OHISHIEriko MAEDASachiko ICHIHARAYoshiki ETOHMitsuhiro HAMASAKIKoichi MURAKAMIShigeyuki TAKENAKAKazumi HORIKAWA
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2013 Volume 30 Issue 2 Pages 132-135

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Abstract
We compared the combination of “Campylo-Preston/225” and “Cica-Immunotest Campylobacter II” with the combination of the method of conventional enrichment methods and “Cica-Immunotest Campylobacter II” for the detection of Campylobacter jejuni/coli. We determined the presence of the pathogens in 52 samples consisting of chicken meat, beef liver and horse meat. With the combination of “Campylo-Preston/225” and “Cica-Immunotest Campylobacter II,” 26 of the 52 samples (50.0%) tested positive for C. jejuni/coli. On the other hand, the combination of the method of conventional enrichment methods and “Cica-Immunotest Campylobacter II” resulted in 18 (34.6%) of the samples testing positive for the bacteria. The results obtained with both combinations of methods were significantly different (p<0.01). For both methodologies, preculture for 48 hr was more effective than preculture for 24 hr. The combination of “Campylo-Preston/225” and “Cica-Immunotest Campylobacter II” showed time-saving potential for the detection of Campylobacter jejuni/coli.
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© 2013 Japanese Society of Food Microbiology
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