Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Methods
Evaluation of Bioluminescent Enzyme Immunoassay: BLEIA to Detect Norovirus in Environmental Swabs
Rikito SHIMOMURADaisuke SHIMIZUHiroshi NAKAGAWA
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2015 Volume 32 Issue 4 Pages 219-223

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Abstract

We swabbed surfaces to detect the presence of Norovirus (NV) using bioluminescent enzyme immunoassay (BLEIA) and to assess the accuracy and usefulness of this method compared with the standard method of real-time PCR. All samples obtained were assessed via both methods. We determined that BLEIA was not only accurate and effective but also cheaper than real-time PCR. Moreover, the simultaneous processing of a large number of samples was easier using BLEIA. Therefore, we recommend the use of BLEIA to rapidly detect NV on a plethora of surfaces.

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© 2015 Japanese Society of Food Microbiology
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