2018 Volume 35 Issue 3 Pages 143-148
Heat-resistant microorganisms can cause food deterioration even after heat sterilization. Generally, heat-resistant microorganisms are screened by cultivating heat-treated samples. However, biased cultivation conditions can inhibit comprehensive isolation of these microorganisms. EMA-PCR-DGGE is considered a comprehensive detection method to selectively analyze viable microbial flora that eliminates bias in the cultivation conditions.
In this study, we screened heat-resistant bacteria by analyzing heated samples using EMA-PCR-DGGE. Specifically, the screening was performed on wipe test fluid samples from a fruit canning factory, and the bacteria were isolated by the addition of cultivation conditions on the screened strains.
We successfully isolated Micromonospora sp. and Propionibacterium sp. The evaluation of heat resistance in these bacteria revealed that Micromonospora sp. was indeed heat resistant; thus, the validity of this method for screening heat-resistant bacteria was confirmed.
