2020 Volume 37 Issue 2 Pages 69-74
We compared the detection rates for the prevalence of Campylobacter jejuni serotypes in Japanese samples by the conventional serotyping method (the Penner serotyping system) and a novel multiplex PCR genotyping method based on the Penner system. A total of 421 C. jejuni isolates from Tokyo (103 isolates from sporadic diarrheal patients and 318 isolates from food poisoning specimens) were examined. The total detection rates were 37.5% (158/421) by serotyping and 94.8% (399/421) by genotyping. By genotyping, the main serogroups detected were B, (HS: 2) 21.1% (89/421); D, (HS: 4 complex) 15.4% (65/421); G, (HS: 8,17) 9.0% (38/421); R, (HS: 23,36,53) 8.6% (36/421); and A, (HS: 1,44) 7.1% (30/421). Furthermore, 10 major serotypes covered 83.4% of all samples (351/421). However, 11 isolates were untypable by both serotyping and genotyping methods, and 11 other isolates serotyped as Z (HS: 38) were not typed by genotyping. Nevertheless, this novel genotyping method is effective and will contribute to updating and improving epidemiological data on C. jejuni infection and food poisoning.
