Rapid Test for Coliforms in Cow Milk and Milk Beverage Using Petrifilm^TM Rapid Coliform Count Plate

Abstract

In this study, we examined rapid assay for coliforms test (presumptive test) using Petrifilm^TM RCC plates for cow milk and milk beverage. The study was conducted on 50 different coliforms and we checked how many hours the RCC plate could determine the coliforms using desoxycholate agar medium (20±2 hr incubation) as a control.

As a result, it was confirmed that 12 hr incubation on RCC plates was equivalent to make a positive determination of the coliforms presumptive test when freshly cultured bacteria were used. However injured bacteria were not considered. Further investigation of applying real food samples is needed.