Evaluation of a Chromogenic Agar, SMID Agar (bioMerieux) for Isolation of Salmonella
1995 Volume 12 Issue 1 Pages 43-49
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1995 Volume 12 Issue 1 Pages 43-49
A chromogenic agar medium, the SMID (Salmonella Detection and Identification) agar (bioMerieux, France), which detects the ability of Salmonella to acidify glucoronate and produce no β-galactosidase, was compared with another chromogenic medium, the Rambach agar, and a conventional medium, the MLCB agar, using 30 Salmonella isolates including 23 serovars and 130 strains other than Salmonella. Also, a comparative study was carried out with unpasteurized liquid eggs, minced chicken meats, and diarrheal specimens to isolate Salmonella.
Although the SMID and Rambach agars were not as selective as the MLCB agar, the former two agars were more specific than the latter. In addition, the specificity of the SMID agar was shown to be higher than that of the Rambach agar on which some strains of serovars Typhi, Enteritidis, and Hadar of Salmonella produced unspecific coloreless colonies. The use of the SMID agar may markedly reduce the number of biochemical tests and times to achieve the identification of Salmonella.
