Evaluation of X-GAL-MUG Test for the Detection of Total Coliform

Bacteria and Escherichia coli in Drinking Water

Abstract

Availability of 5-bromo-4-chloro 3-indoly-β-D-garactopyranoside-MUG (X-GAL-MUG) for total coliform bacteria and Escherichia coli has been evaluated in comparison with o-nitrophenyl-β-D-garactopyranoside-4-methyl-umbelliferyl-β-D-glucuronide (MMO-MUG) method and traditional lactose fermentation method. With 200 drinking well water samples, we obtained the coincidence of in the 90% of results obtained by X-GAL-MUG and MMO-MUG method, and also 93% by X-GAL-MUG and traditional lactose fermentation method. these seen to indicate the usefulness of X-GAL-MUG method for the detection of total coliform bacteria and E. coli in drinking well water.