Abstract
An assay to detect halophilic non-sporeforming anaerobic Gram nagative rods in seafish and shellfish on the market (Tapes japonica, Meretrix lusoria, Crassostrea gigas, Babyronia japonica, Iefteye flounder, Pandalus borealis, Metapenaeus joyneri, Sardinops melanosticta) using an enrichment culture with 2% NaC1 and simple plate agar with 2% NaC1 culture isolationprocedure was staudied. By the enrichment culture method, halophilic non-spore forming anaerobic Gram negative rode were detected in 50.8% of the seafish and shellfish. The detection rate of halophilic non-sporeforming anaerobic Gram negative rods from shellfish in the winter season was higher than that in summer (p<0.00 1 and p<0.029). Concentration of halophilic non-sporeforming anaerobic Gram negative rods in the alimentary canal of shellfish detected 4.3×103-18.8×103cfu/g in winter season.
We compared 45 strains of halophilic anaerobic non-sporeforming Gram negative rods isolated from the sea shellfish with the Haloanaerobium praevalens DSM 2228 strain. All of these strains were obligate anaerobic Gram negative, non-sporeforming bacteria which proliferated optimally atapproximately 2-3% or ≥ 5% salt. DNA base composition was 26.9-33.2 M% guanosine plus cytosine. This strain of these isolates have negligible DNA homology with the H. praevalens DSM 2228 strain described previously. Acetic acid, butyric acid and propionic acid were the major glucose fermentation end products formed. Glucose, fructose, inositol, ribose, maltose and mannose were fermented. A number of additional biochemical and physiological tests were performed. On the basis of those characteristics, isolates were identified as the new Haloanaerobium species, H. butyricum JCM 9809.
