Abstract
A method for the detection and enumeration of enterohemorhagic Escherichia coli (EHEC) O157 in various samples has been reported consisting of incubation on EC medium with novobiocin at 42°C for 18hr, and is recognized by the Japanese Ministry of Welfare as the official method on July 18, 1996
Some strains of EHEC can't be grown in this official method. The present study has shown that all of EHEC in TS broth with novobiocin grew better at 44.0°C than at 44.5°C and 42°C, and its strains were enough to be incubated the short time (3-6 hr) for the increase.
In the immunomagnetic separation method, which is indispensable for the improvement of the detection rate, the stable high recovery percentage was obtained at ten minites of reaction. This combination method was shown to allow for very rapid and accurate deter-mination.
