Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Evaluation of Selective ES Salmonella Medium for Isolating Salmonella from Food and Feed
Hiroshi NAKAGAWAManami ARAIMasaya KADONOMasanari IKEDOOsamu KOMASTUTakeshi ITOHSenzo SAKAI
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1999 Volume 16 Issue 4 Pages 249-255

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Abstract

The purpose of this study was to evaluate the isolated media of Salmonella and to determine the usefulness of practices used for isolation of Salmonella from food and feed.
ES Salmonella medium was based on the use of X-GAL enzyme, and compared to other isolated media for detection of Salmonella. A total of 47 Salmonella and various gramnegative and gram-positive bacteria were used for evaluation of the media with testing by the method of Miles and Misra.
Salmonella Enteritidis was put in liquid eggs, broilers and human feces to compare detection of Salmonella Enteritidis, and the contamination of Salmonella was examined for isolation from those by the selective medium.
ES Salmonella medium was as effective or better than other commercial selective media such as DHL, MLCB, BG and SMID media for isolation of Salmonella.
ES Salmonella medium was therefore considered effective for isolation of Salmonella from food and feed, but not from the samples of human feces.

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