Abstract
A rapid assay was developed for detection of small numbers ofCampylobactercells in food samples. The intergenic sequence between the 16S rRNA gene ofCampylobacter jejuniwas amplified and characterized with a triple primer or semi-nested primer approach . The detection limit as determinated by ethidium bromide staining of amplification products on agarose gels was 10 bacteria or fewer in artificially contaminated samples with the seminested PCR assay.
