Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
Investigation of the Actual Conditions of Pathogenic Bacteria among Imported Fresh Vegetables and Fruits
Minoru MURASETsutomu MIYATAHiroko KIMATAManabu KUROKAWA
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2002 Volume 19 Issue 2 Pages 71-75

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Abstract

We examined 82 imported raw vegetables and 14 imported raw fruits for contamination with Salmonella, Enterohemorrhagic Escherichia coli O 157: H 7, and Listeria. Of 12 bean sprout samples, one was contaminated with L. monocytogenes serotype 1/2a, and two with L. innocua. One of two kiwi fruit samples was contaminated with L. seeligeri. Aeromonas caviae was isolated from one of five young field pea samples. One of three alfalfa samples was contaminated with Enteropathogenic E. coli 08. Three months later, we examined an additional 10 imported bean sprout samples obtained from the store that had sold the L. monocytogenes-contaminated bean sprouts described above. L. monocytogenes serotype 1 /2a was isolated from three samples, and L. innocua from one. The AFLP technique (Amplified Fragments Length Polymorphism) was used for genetic analysis of four isolates of L. mono-cytogenes serotype 1/2a and no homology was found among the isolates. The high L. monocytogenes-contamination rate for bean sprouts indicates poor hygiene control in growth, harvest, washing, distribution, and storage of the product. The washing and handling of imported raw vegetables and fruits should be improved to prevent food-borne disease.

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