Abstract
DIASALM was tested for its suitability for the simple, rapid screening and examination of typical and atypical Salmonella. In an additional recovery test by the DIASALM method that used minced chicken meat, 8 strains of atypical Salmonella including S. Enteritidis not producing hydrogen sulfide, and lysine-negative S. Chester were successfully recovered. Using the DIASALM method and the FSIS method which shows a high isolation rate, 32 samples of minced chicken meat were examined. As a result, 17 (53.1%) of the samples were judged positive by both examination methods. The detection sensitivities of these 2 exmination methods were the same, but screening of bacteria with the DIASALM method achieved significantly better results than with the FSIS method. This was because, using the DIASALM method, pure Salmonella could be incubated, whereas significant contamination with other bacteria occurs with the FSIS method. If the DIASALM is used, the latex agglutination test after incubation for 24 hours could rapidly confirm Salmonella. DIASALM is an excellent examination method for epidemiological investigations and the detection of typical and atypical Salmonella can be rapidly evaluated.
