Abstract
The enzymatic assay (PPi assay) for inorganic pyrophosphate (PPi) released from nucleotides by polymerase chain reaction (PCR) was compared with the electrophoresis assay in identification of food poisoning bacteria. In this study, 15 species of bacteria and 7 kinds of commercial primers were used. The amplicons and PPi produced by PCR were confirmed by electrophoresis and PPi assay, respectively. In comparison, the PPi assay showed false-positive in bacteria having non-specific genes, using 4 kinds of primers, but showed positive in all bacteria having specific genes, using 7 kinds of primers.
These results suggested that PPi assay could be a simple and effective method for rapid identification of food poisoning bacteria.
