2007 Volume 42 Issue 4 Pages 225-228
Among the four types of betanodaviruses, redspotted grouper nervous necrosis virus (RGNNV) has the highest optimum temperature (25-30°C) for its multiplication. We tested 16 RGNNV isolates for their temperature sensitivity in cultured cells and demonstrated that their upper temperature limits ranged from less than 30°C to 35°C. At the temperatures over the upper limits, viral RNA replication was inhibited similarly. These results indicate that temperatures mainly affect RNA replication or earlier virus multiplication processes. The incompetence of betanodaviruses at 37°C suggests their avirulence in human.