2009 Volume 44 Issue 2 Pages 94-97
In this study, we have developed a DNA vaccine encoding viral envelope protein VP28 of penaeid rod-shaped DNA virus (PRDV, = WSSV). Protective efficacy of the DNA vaccine against PRDV was confirmed at 7 days post vaccination in kuruma shrimp Penaeus japonicus. The VP28 transcript derived from the vaccine was detected in various tissues at 1, 3 and 7 days post vaccination. Moreover, notable up-regulated expression of Rab7, penaeidin, lysozyme and crustin genes was observed upon DNA vaccination. These results suggest that the DNA vaccine significantly increased the protection and innate immune responses in kuruma shrimp against PRDV.