Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Research Articles
Persistent Expression of Shrimp-virus Antigens in Two Insect Cell Lines Challenged with Two Shrimp Viruses
Anuwat SritonNipaporn KanthongWarachin GangnonngiwSiriporn SriurairatanaSukathida UbolTimothy W. Flegel
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JOURNAL FREE ACCESS

2009 Volume 44 Issue 2 Pages 86-93

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Abstract

Experiments with crustacean viruses are hampered by lack of susceptible continuous cell lines. To overcome this problem, immortal mosquito and lepidopteran cell lines were both separately challenged with a shrimp DNA virus (white spot syndrome virus: WSSV, = PRDV) and RNA virus (yellow head virus: YHV) followed by serial, split-passage with immunohistochemical monitoring by confocal laser microscopy using labeled monoclonal antibodies to shrimp viral antigens. Stable, immortal cultures with 100% of the cells expressing shrimp-virus antigens were obtained, although the infected cells appeared grossly normal by phase contrast microscopy. Nor did they show any ultrastructural modifications characteristic of the challenge viruses. These persistently-expressing insect cell cultures were stable and could be continuously passaged, stored and revived as required. Since disparate viruses and insect cells were used, this appears to be a generic process that may be applicable to other shrimp viruses as well.

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© 2009 The Japanese Society of Fish Pathology
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