Abstract
Cyprinid herpesvirus 3 (CyHV-3) is the cause of a lethal disease named as koi herpesvirus disease (KHVD) in carp worldwide. Even though CyHV-3 has been found in China, little is known about the variety of CyHV-3 genotypes distributing in this area. In the present study, to improve the sensitivity of current genotyping approaches, a nested-PCR method was constructed based on the duplex PCR reported previously. The nested-PCR targeting the markers I/II loci was used in genotype identification of CyHV-3 infected fish in Guangdong Province in 2010-2015 and Henan Province in 2013. We detected 3 genotypes including an Asian dominant type (I++ II+ ) and two emerging genotypes in East Asian and South-East Asian regions (I++ II− and I++ II+Δ). I++ II− was the predominant genotype in this study. Two CyHV-3 variants, ZS410 and HZ419 isolates, were successfully isolated in cell culture and exhibited the genotype of I++ II− and I++ II+Δ, respectively. This is the first report of both I++ II− and I++ II+Δ genotypes isolated in China. These data can contribute consideration and exploration of mode of CyHV-3 spread in China.
