Mortality of Pond-cultured Litopenaeus vannamei Associated with Acute Hepatopancreatic Necrosis Disease (AHPND) and White Spot Syndrome Virus (WSSV) Infection in the Philippines

Leobert D. de la Peña; Nikko Alvin R. Cabillon; Edgar C. Amar; Demy D. Catedral; Roselyn C. Usero; Joseph P. Faisan, Jr.; Joey I. Arboleda; Wilberto D. Monotilla; Adelaida T. Calpe; Dalisay D. G. Fernandez; Cynthia P. Saloma

doi:10.3147/jsfp.52.38

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Mortality of Pond-cultured Litopenaeus vannamei Associated with Acute Hepatopancreatic Necrosis Disease (AHPND) and White Spot Syndrome Virus (WSSV) Infection in the Philippines

Leobert D. de la Peña, Nikko Alvin R. Cabillon, Edgar C. Amar, Demy D. Catedral, Roselyn C. Usero, Joseph P. Faisan, Jr., Joey I. Arboleda, Wilberto D. Monotilla, Adelaida T. Calpe, Dalisay D. G. Fernandez, Cynthia P. Saloma

Author information

Leobert D. de la Peña
Aquaculture Department, Southeast Asian Fisheries Development Center
Nikko Alvin R. Cabillon
University of Highlands and Islands-Scottish Association for Marine Science
Edgar C. Amar
Aquaculture Department, Southeast Asian Fisheries Development Center
Demy D. Catedral
Aquaculture Department, Southeast Asian Fisheries Development Center
Roselyn C. Usero
Negros Prawn Producers Cooperative Analytical and Diagnostic Laboratory
Joseph P. Faisan, Jr.
Aquaculture Department, Southeast Asian Fisheries Development Center
Joey I. Arboleda
Aquaculture Department, Southeast Asian Fisheries Development Center
Wilberto D. Monotilla
Philippine Genome Center, University of the Philippines Diliman
Adelaida T. Calpe
Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development
Dalisay D. G. Fernandez
Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development
Cynthia P. Saloma
Philippine Genome Center, University of the Philippines Diliman

Keywords: AHPND, Vibrio parahaemolyticus, WSSV, Litopenaeus vannamei, dual infection

JOURNAL FREE ACCESS

2017 Volume 52 Issue 1 Pages 38-41

DOI https://doi.org/10.3147/jsfp.52.38

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Abstract

Mortalities of up to 60% were observed in pond-cultured Litopenaeus vannamei in Bohol, Philippines. Histopathological examination revealed typical acute hepatopancreatic necrosis disease (AHPND) pathology. PCR test generated 1,269 bp and 230 bp amplicons confirmative for the toxin-producing AHPND strain of Vibrio parahaemolyticus among shrimp sampled from eight ponds. The same samples were subjected to PCR analyses for the presence of other viruses, namely WSSV, IHHNV, IMNV, and TSV. The samples were negative for the viruses except WSSV, which was detected after one-step PCR in six out of eight ponds. These results suggested that shrimp were infected dually with AHPND V. parahaemolyticus and WSSV.

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