Abstract
The SMART established first to selectively isolate plant pathogens was applied to develop a selective medium for isolation of fish-pathogenic Edwardsiella tarda. The SMART medium selectively allowed the growth of E. tarda only when a mixture of E. tarda and other fish pathogens was inoculated onto the medium. The medium also enabled the direct culturing of E. tarda from diseased Japanese eel Anguilla japonica. However, two other strains from eel intestinal contents and diseased tilapia did not grow on the medium. Most of E. tarda strains from diseased marine fishes also did not grow. Therefore, the medium made by SMART was applicable to selectively isolate E. tarda from diseased Japanese eel.
