Virucidal Effect of Disinfectants against Erythrocytic Inclusion Body Syndrome (EIBS) Virus Piscine orthoreovirus 2

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The virucidal effect of disinfectants on Piscine orthoreovirus 2 (PRV-2), a causative agent of erythrocytic inclusion body syndrome (EIBS), was assessed through challenge experiments with EIBS-naïve juvenile coho salmon Oncorhynchus kisutch. ​A 10-fold dilution of PRV-2 virus suspension (1.05 × 10¹⁰ copies/mL of PRV-2 L2 RNA) was completely inactivated by 50 ppm povidone-iodine (15 min), 200 ppm sodium hypochlorite (30 s), or 40% ethanol (30 s). ​A 100-fold dilution of PRV-2 (1.05 × 10⁹ copies/mL) was inactivated by 12.5 ppm povidone-iodine (15 min), or by 25 ppm sodium hypochlorite (30 s); 1,600 ppm benzalkonium chloride (30 s) did not inactivate PRV-2.