抄録
Two hundred nineteen strains of causative organisms of fish vibriosis, which were isolated from various kinds of diseased fish cultured from all over the country, and 29 reference strains were examined by means of ordinary taxonomy, numerical taxonomy, DNA base composition and serological analysis.
One hundred and seventy strains(phenon I, see Fig.1)isolated from coho salmon(Oncorhynchus kisutch), ayu(Pleocoglossus altivelis), eel(Anguilla spp.), yellowtail(Seriola quinqueradiata)and amberjack(Seriola purpurascens)were identified as V. anguillarum. Twenty five strains(phenon II, see Fig.1)were not identified as V. anguillarum, but were closely related to above taxon in many characteristics.These strains were isolated from rainbow trout(Salmo gairdnerii), yamabe(O.masou var.ishikawae)and amago(O. rhodurus var. macrostomus)reared in fresh water. Twenty one strains isolated from ayu, yellowtail, red sea bream(Chrysophrys major)and anchovy(Engraulis japonica)could not be identified as any species in genus Vibrio, and many differences were observed among them. The other 3 strains isolated from rainbow trout and ayu were identified as Aeromonas spp.
One hundred and ninety two strains out of 195 strains identified as V.anguillarum and its related organisms(phenon I and II), were distinguished into three serological groups according to the serological analysis of the somatic antigens.Serotype J-O-1 was comprised of 100 strains of V. anguillarum and 25 strains related to the species. One hundred and ten strains out of the above 125 strains were originally isolated from ayu, eel, rainbow trout, amago and yamabe reared in fresh water.Serotype J-O-2 was comprised of 11 strains isolated from ayu and eel reared in fresh or salt water.Serotype J-O-3 was comprised of 56 strains isolated from ayu, coho salmon, yellowtail and amberjack.Except the 10 strains from ayu in fresh water, 46 strains were isolated from the fish reared in sea water.
