1981 年 15 巻 3-4 号 p. 315-318
As red spot disease of eels (Pseudomonas anguilliseptica infection) has occurred restrictedly in Japan so far, a brief histroy of investigations of the disease was given before entering the main issue.
In order to find practicable methods for artificial infection in Japanese eels (Anguilla japomica), injection, oral administration, addition, and immersion methods were tested in this study. As the results, the three methods except the oral administration were demonstrated to cause a mortal infection in the experimental animal. In the addition method, eels were required to be kept in salt containing water in order to be successfully infected. In the immersion method, a pre-immersion in 7 % saline for 10 min or 10 % saline for 5 min was necessary to bring a definite result.
The addition technique was thought to be most applicable for challenge test in vaccinated and emaciated eels.